Title: Structural elucidation of the oncomiR-1 RNA
Abstract: MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression. To maintain proper microRNA expression levels, the enzymatic processing of primary and precursor microRNA elements must be strictly controlled. However, the molecular determinants underlying this strict regulation of microRNA biogenesis are not fully understood. We are investigating the differential processing of oncomiR-1, a polycistronic primary microRNA that is overexpressed in many cancers. Using a combination of solution NMR spectroscopy, biochemical, and biophysical approaches, we are examining the extent to which (1) RNA structure and dynamics, and (2) RNA remodeling by protein partners, contribute to the regulation of oncomiR-1 processing.Â